Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX60342 - Provider product page
- Provider
- GeneTex
- Product name
- RPB1 antibody [GT9010]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of nuclear extracts (25 £gg) from HeLa cells using Pol II antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells using Pol II antibody and with DAPI. Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Pol II antibody at a dilution of 1:500 (red). The nuclei were stained with DAPI.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with HeLa nuclear extract and either 2 £gg of PolII antibody or control mouse IgG. The precipitated DNA was detected by QPCR with primer set targeting to ACTB promoter or Sat2.
