664906
antibody from BioLegend
Targeting: POLR2A
POLR2, POLRA, RPB1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 664906 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#664906, RRID:AB_2565554
- Product name
- Purified anti-RNA Polymerase II RPB1
- Antibody type
- Monoclonal
- Antigen
- Purified protein
- Description
- RPB1 is the catalytic and largest component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with RPB2, the second largest subunit. Polymerase II (Pol II) is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relatively to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft, and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single DNA template strand of the promoter is positioned within the central active site cleft of Pol II. Then, a bridging helix emanates from RPB1 and crosses the cleft near the catalytic site, which acts as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations during each neuocleotide addition. This promotes translocation of Pol II. Pol II moves on the template during transcription elongation. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II's largest subunit (RPB1), which serves as a platform for assembling factors that regulate transcription initiation, elongation, termination, and mRNA processing. It can act as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, being able to conform as both a replicate and transcriptase for the viral RNA circular genome.
- Reactivity
- Human, Mouse, Yeast
- Host
- Mouse
- Antibody clone number
- 8WG16
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Western blot analysis of E. coli (BL21) (lane 1), yeast (saccharomyces cerevisiae) (lane 2) and Hela (lane 3). Anti-RPB1 antibody (clone 8WG16) was used and followed by HRP anti-mouse IgG antibody.
- Conjugate
- Unconjugated