Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- STJ91031 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-Phospho-Rpb1 (S1619) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- 1560-1640 aa
- Isotype
- IgG
- Vial size
- 200μl,50μl,100μl
- Concentration
- 1 mg/ml
- Storage
- Store at -20°C, and avoid repeat freeze-thaw cycles.
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA