920204

antibody from BioLegend

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

920204

Provider

BioLegend

Proper citation

BioLegend Cat#920204, RRID:AB_2616695

Product name

Purified anti-RNA Polymerase II RPB1

Antibody type

Monoclonal

Antigen

The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

H5

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot of clone H5. Lane 1: Daudi cell lysate; Lane 2: Jurkat cell lysate; Lane 3: HeLa cell lysate; Lane 4: Molecular weight marker. The blot was incubated with clone H5 at 1 µg/ml for 24 hours at 4°C, followed by incubation with HRP-anti mouse IgM secondary antibody.

Conjugate

Unconjugated