Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB30191 - Provider product page
- Provider
- Abnova Corporation
- Product name
- POLR2A CTD repeat (phospho S2) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human POLR2A CTD repeat (phospho S2).
- Antigen sequence
Y-Sp-P-T-S-P-S
- Isotype
- IgG
- Storage
- Store at -20°C or lower.Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of MCF-7 cell lysates with POLR2A CTD repeat (phospho S2) polyclonal antibody (Cat # PAB30191).Lane 1: POLR2A CTD repeat (phospho S2) polyclonal antibody (Cat # PAB30191) pre-incubated with non-phospho-peptideLane 2: POLR2A CTD repeat (phospho S2) polyclonal antibody (Cat # PAB30191) pre-incubated with POLR2A CTD repeat (phospho S2) phospho-peptideLane 3: POLR2A CTD repeat (phospho S2) polyclonal antibody (Cat # PAB30191) pre-incubated with POLR2A CTD repeat (phospho S5) phospho-peptideLane 4: POLR2A CTD repeat (phospho S2) polyclonal antibody (Cat # PAB30191)