PAB30192

antibody from Abnova Corporation

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB30192

Provider

Abnova Corporation

Product name

POLR2A CTD repeat (phospho S5) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against partial synthetic phosphopeptide of human POLR2A CTD repeat (phospho S5).

Antigen sequence

Y-S-P-T-Sp-P-S


Isotype

IgG

Storage

Store at -20°C or lower.Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MCF-7 cell lysates with POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192).Lane 1: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192)Lane 2: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192) pre-incubated with phospho-peptideLane 3: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192) pre-incubated with non-phospho-peptideLane 4: POLR2A CTD repeat (phospho S5) polyclonal antibody (Cat # PAB30192) pre-incubated with generic [pS] peptide