V7525

antibody from NSJ Bioreagents

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

V7525

Provider

NSJ Bioreagents

Product name

POLR2A Antibody / RNA polymerase II subunit RPB1 / Phosphorylated Serine 5

Antibody type

Monoclonal

Description

PEG precipitation

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)

Storage

Store the phospho-RNA polymerase II antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

References

Submitted references

Highly asynchronous and asymmetric cleavage divisions accompany early transcriptional activity in pre-blastula medaka embryos.

Kraeussling M, Wagner TU, Schartl M
PloS one 2011;6(7):e21741

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Prolyl isomerase Pin1 shares functional similarity with phosphorylated CTD interacting factor PCIF1 in vertebrate cells.

Yunokuchi I, Fan H, Iwamoto Y, Araki C, Yuda M, Umemura H, Harada F, Ohkuma Y, Hirose Y
Genes to cells : devoted to molecular & cellular mechanisms 2009 Sep;14(9):1105-18

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Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation.

Li OT, Chan MC, Leung CS, Chan RW, Guan Y, Nicholls JM, Poon LL
PloS one 2009 May 21;4(5):e5658

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Hyperphosphorylation of RNA polymerase II in response to topoisomerase I cleavage complexes and its association with transcription- and BRCA1-dependent degradation of topoisomerase I.

Sordet O, Larochelle S, Nicolas E, Stevens EV, Zhang C, Shokat KM, Fisher RP, Pommier Y
Journal of molecular biology 2008 Sep 5;381(3):540-9

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FLASH acts as a co-activator of the transcription factor c-Myb and localizes to active RNA polymerase II foci.

Alm-Kristiansen AH, Saether T, Matre V, Gilfillan S, Dahle O, Gabrielsen OS
Oncogene 2008 Aug 7;27(34):4644-56

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Purification of elongating RNA polymerase II and other factors from yeast chromatin.

Svejstrup JQ, Petrakis TG, Fellows J
Methods in enzymology 2003;371:491-8

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HepG2, K562, and SK-BR-3 cell lysate with phospho-RNA polymerase II antibody (clone CTD 8A7). Routinely observed molecular weight: 200-250 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HepG2, K562, and SK-BR-3 cell lysate with phospho-RNA polymerase II antibody (clone CTD 8A7). Routinely observed molecular weight: 200-250 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.