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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- NBP1-49546 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49546, RRID:AB_10011602
- Product name
- Rabbit Polyclonal RNA Polymerase II/POLR2A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
- Reactivity
- Human, Chicken/Avian, Drosophila, Yeast
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination.
Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.
Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.
RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3' end processing.
Kamieniarz-Gdula K, Gdula MR, Panser K, Nojima T, Monks J, Wiśniewski JR, Riepsaame J, Brockdorff N, Pauli A, Proudfoot NJ
Molecular cell 2019 Apr 4;74(1):158-172.e9
Molecular cell 2019 Apr 4;74(1):158-172.e9
Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.
Hsin JP, Xiang K, Manley JL
Molecular and cellular biology 2014 Jul;34(13):2488-98
Molecular and cellular biology 2014 Jul;34(13):2488-98
Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.
Allepuz-Fuster P, Martínez-Fernández V, Garrido-Godino AI, Alonso-Aguado S, Hanes SD, Navarro F, Calvo O
Nucleic acids research 2014 Dec 16;42(22):13674-88
Nucleic acids research 2014 Dec 16;42(22):13674-88
RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3' end processing.
Hsin JP, Sheth A, Manley JL
Science (New York, N.Y.) 2011 Nov 4;334(6056):683-6
Science (New York, N.Y.) 2011 Nov 4;334(6056):683-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Dot blot analysis of RNA pol II CTD pT4 using phosphorylated and non-phosphorylated peptides.
