PA5-28087

antibody from Invitrogen Antibodies

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28087

Provider

Invitrogen Antibodies

Product name

POLR2A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, H1299, HepG2, Raji.

Concentration

0.72 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using POLR2A Polyclonal Antibody (Product # PA5-28087). Sample (30 µg of whole cell lysate). Lane A: 293T .5% SDS PAGE. POLR2A Polyclonal Antibody (Product # PA5-28087) diluted at 1:2,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-POLR2A Polyclonal Antibody (Product # PA5-28087) and ~300kDa band corresponding to POLR2A was observed across cell lines tested. Whole cell extracts (40 µg lysate) of HeLa (Lane 1) and A549 (Lane 2) were electrophoresed using NuPAGE® 10% Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock® Mini-Cell and XCell II® Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).