NB100-1805

antibody from Novus Biologicals

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1805

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1805, RRID:AB_10001499

Product name

Rabbit Polyclonal RNA Polymerase II/POLR2A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. RNA Polymerase 2 Phosphospecific [Ser2]

Reactivity

Human, Mouse, Yeast

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

ATR Protects the Genome against R Loops through a MUS81-Triggered Feedback Loop.

Matos DA, Zhang JM, Ouyang J, Nguyen HD, Genois MM, Zou L
Molecular cell 2020 Feb 6;77(3):514-527.e4

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Targeting CDK9 and MCL-1 by a new CDK9/p-TEFb inhibitor with and without 5-fluorouracil in esophageal adenocarcinoma.

Tong Z, Mejia A, Veeranki O, Verma A, Correa AM, Dokey R, Patel V, Solis LM, Mino B, Kathkuda R, Rodriguez-Canales J, Lin SH, Krishnan S, Kopetz S, Blum M, Ajani JA, Hofstetter WL, Maru DM
Therapeutic advances in medical oncology 2019;11:1758835919864850

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Antitumor effects of cyclin dependent kinase 9 inhibition in esophageal adenocarcinoma.

Tong Z, Chatterjee D, Deng D, Veeranki O, Mejia A, Ajani JA, Hofstetter W, Lin S, Guha S, Kopetz S, Krishnan S, Maru D
Oncotarget 2017 Apr 25;8(17):28696-28710

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Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.

Allepuz-Fuster P, Martínez-Fernández V, Garrido-Godino AI, Alonso-Aguado S, Hanes SD, Navarro F, Calvo O
Nucleic acids research 2014 Dec 16;42(22):13674-88

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Identification of novel inhibitors of human immunodeficiency virus type 1 replication by in silico screening targeting cyclin T1/Tat interaction.

Hamasaki T, Okamoto M, Baba M
Antimicrobial agents and chemotherapy 2013 Mar;57(3):1323-31

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KRIBB11 inhibits HSP70 synthesis through inhibition of heat shock factor 1 function by impairing the recruitment of positive transcription elongation factor b to the hsp70 promoter.

Yoon YJ, Kim JA, Shin KD, Shin DS, Han YM, Lee YJ, Lee JS, Kwon BM, Han DC
The Journal of biological chemistry 2011 Jan 21;286(3):1737-47

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Sex-induced silencing defends the genome of Cryptococcus neoformans via RNAi.

Wang X, Hsueh YP, Li W, Floyd A, Skalsky R, Heitman J
Genes & development 2010 Nov 15;24(22):2566-82

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CDKN1C negatively regulates RNA polymerase II C-terminal domain phosphorylation in an E2F1-dependent manner.

Ma Y, Chen L, Wright GM, Pillai SR, Chellappan SP, Cress WD
The Journal of biological chemistry 2010 Mar 26;285(13):9813-22

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RNA polymerase II C-terminal domain phosphorylation patterns in Caenorhabditis elegans operons, polycistronic gene clusters with only one promoter.

Garrido-Lecca A, Blumenthal T
Molecular and cellular biology 2010 Aug;30(15):3887-93

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Posttranslational regulation of CIRCADIAN CLOCK ASSOCIATED1 in the circadian oscillator of Arabidopsis.

Yakir E, Hilman D, Kron I, Hassidim M, Melamed-Book N, Green RM
Plant physiology 2009 Jun;150(2):844-57

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CpG-island promoters drive transcription of human telomeres.

Nergadze SG, Farnung BO, Wischnewski H, Khoriauli L, Vitelli V, Chawla R, Giulotto E, Azzalin CM
RNA (New York, N.Y.) 2009 Dec;15(12):2186-94

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Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II.

Nordick K, Hoffman MG, Betz JL, Jaehning JA
Eukaryotic cell 2008 Jul;7(7):1158-67

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Triptolide-induced transcriptional arrest is associated with changes in nuclear substructure.

Leuenroth SJ, Crews CM
Cancer research 2008 Jul 1;68(13):5257-66

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Hela whole cell lysate (50 ug each lane) loaded and probed by anti-RNA polymerase II Antibody. NB100-1805 was pre-incubated without peptide (-), with non-phosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.08 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-RNA Polymerase II (S2). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Mouse Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-RNA Polymerase II (S2). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Analysis of POLR2A in MCF-7 xenograft using anti-POLR2A [p Ser2] antibody. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:500 (

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Human ovarian carcinoma. Antibody: Affinity purified rabbit antiPhospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-PhosphoRNA Polymerase II (S2) used at a dilution of 1:200 (1ug/ml). Detection: DAB