TA503259

antibody from Invitrogen Antibodies

Targeting: DHFR2 DHFRL1, DHFRP4, FLJ16119

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503259

Provider

Invitrogen Antibodies

Product name

DHFRL1 Monoclonal Antibody (OTI1E2), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1E2

Vial size

100 µL

Concentration

0.73 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFRL1 (RC208452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFRL. Positive lysates LY406051 (100 µg) and LC406051 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFRL1 (RC208452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFRL. Positive lysates LY406051 (100 µg) and LC406051 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-DHFRL1 monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-DHFRL1 mouse monoclonal antibody (TA503259) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFRL1(RC208452).