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antibody from Invitrogen Antibodies
Targeting: EIF3A
EIF3, eIF3-p170, eIF3-theta, EIF3S10, KIAA0139, TIF32
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31296 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF3a Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji. Predicted reactivity: Mouse (99%), Rat (100%), Xenopus laevis (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF3A in 100 ng of 24 kDa recombinant EIF3A using an EIF3A polyclonal antibody (Product # PA5-31296) at a dilution of 1:90,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eIF3a Polyclonal Antibody (Product # PA5-31296). Sample (30 µg of whole cell lysate). Lane A: Raji. 5% SDS PAGE. eIF3a Polyclonal Antibody (Product # PA5-31296) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF3A in methanol-fixed A431 cells using an EIF3A polyclonal antibody (Product # PA5-31296) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of eIF3a was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF3a Polyclonal Antibody (Product # PA5-31296) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using EIF3A (Product # PA5-31296) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
