RZ1126

antibody from NSJ Bioreagents

Targeting: EIF3A EIF3, eIF3-p170, eIF3-theta, EIF3S10, KIAA0139, TIF32

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RZ1126

Provider

NSJ Bioreagents

Product name

Zebrafish Eif3a Antibody / Eukaryotic translation initiation factor 3 subunit A

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Zebrafish

Host

Rabbit

Vial size

100 ug

Concentration

Lyophilized

Storage

After reconstitution, the Zebrafish Eif3a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of Eif3a protein using Zebrafish Eif3a antibody and 1) whole female zebrafish and 2) whole male zebrafish tissue lysate. Predicted molecular weight ~167 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish testis tissue with Zebrafish Eif3a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish kidney tissue with Zebrafish Eif3a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish colon tissue with Zebrafish Eif3a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish brain tissue with Zebrafish Eif3a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.