Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-79411 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSPB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPB2 in Lane 1: rat liver tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: HeLa whole cell lysate, Lane 4: COLO320 whole cell lysate, Lane 5: HT1080 whole cell lysate, Lane 6: MCF-7 whole cell lysate using 40-50 µg per well. Sample was incubated with HSPB2 (Product # PA5-79411) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPB2 in, Lane 1: human U20S whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat H9C2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse C2C12 whole cell lysates, Lane 6: mouse heart tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with HSPB2 Polyclonal Antibody (Product # PA5-79411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for HSPB2 at approximately 23 kDa. The expected band size for HSPB2 is at 20 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HSPB2 in HeLa cells. Sample was incubated with HSPB2 polyclonal antibody (Product # PA5-79411).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSPB2 on frozen rat cardiac muscle tissue. Sample was incubated with HSPB2 polyclonal antibody (Product# PA5-79411).