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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-27624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-REEP1/REEP2 Monoclonal Antibody (S326D-29)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S326D-29
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of REEP1/REEP2 in rat brain with 20 µg of sample. The sample was incubated with REEP1/REEP2 monoclonal antibody (Product # MA5-27624) using a dilution of 1:1000 (16 hours at 4°C), and followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:200 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Rat Brain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of REEP1/REEP2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with REEP1/REEP2 monoclonal antibody (Product # MA5-27624) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 and 1:5000. Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) REEP1/2 Antibody, D) Merged image. Magnification: 60x.
