Antibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 12835-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12835-1-AP, RRID:AB_1072935
- Product name
- KDM3A,JMJD1A antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated KDM3A,JMJD1A antibody (Cat. #12835-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.
HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury.
MicroRNA-449a delays lung cancer development through inhibiting KDM3A/HIF-1α axis.
Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice.
NG2/CSPG4, CD146/MCAM and VAP1/AOC3 are regulated by myocardin-related transcription factors in smooth muscle cells.
BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level.
BRG1 Stimulates Endothelial Derived Alarmin MRP8 to Promote Macrophage Infiltration in an Animal Model of Cardiac Hypertrophy.
Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2.
Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer.
Histone Demethylase JMJD1A Promotes Tumor Progression via Activating Snail in Prostate Cancer.
Epigenetic activation of CTGF transcription by high glucose in renal tubular epithelial cells is mediated by myocardin-related transcription factor A.
p300-Mediated Acetylation of Histone Demethylase JMJD1A Prevents Its Degradation by Ubiquitin Ligase STUB1 and Enhances Its Activity in Prostate Cancer.
Histone demethylase JMJD1A promotes expression of DNA repair factors and radio-resistance of prostate cancer cells.
Epigenetic regulation of lung cancer cell proliferation and migration by the chromatin remodeling protein BRG1.
Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.
Acetylation of PHF5A Modulates Stress Responses and Colorectal Carcinogenesis through Alternative Splicing-Mediated Upregulation of KDM3A.
Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.
Epigenetic activation of PERP transcription by MKL1 contributes to ROS-induced apoptosis in skeletal muscle cells.
Impact of histone demethylase KDM3A-dependent AP-1 transactivity on hepatotumorigenesis induced by PI3K activation.
Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.
KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.
JMJD1A promotes tumorigenesis and forms a feedback loop with EZH2/let-7c in NSCLC cells.
Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.
The hypoxia-inducible epigenetic regulators Jmjd1a and G9a provide a mechanistic link between angiogenesis and tumor growth.
A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression.
Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis.
Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen regulates the KSHV epigenome by association with the histone demethylase KDM3A.
Role of the hypoxia-related gene, JMJD1A, in hepatocellular carcinoma: clinical impact on recurrence after hepatic resection.
The JmjC domain-containing histone demethylase KDM3A is a positive regulator of the G1/S transition in cancer cells via transcriptional regulation of the HOXA1 gene.
Upregulation of MiR-155 in nasopharyngeal carcinoma is partly driven by LMP1 and LMP2A and downregulates a negative prognostic marker JMJD1A.
Jumonji domain containing 1A is a novel prognostic marker for colorectal cancer: in vivo identification from hypoxic tumor cells.
Liu W, Wang C, Wang S, Zeng K, Wei S, Sun N, Sun G, Wang M, Zou R, Liu W, Lin L, Song H, Jin Z, Zhao Y
International journal of biological sciences 2021;17(1):188-203
International journal of biological sciences 2021;17(1):188-203
HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury.
Zhao M, Wang S, Zuo A, Zhang J, Wen W, Jiang W, Chen H, Liang D, Sun J, Wang M
Cellular & molecular biology letters 2021 Sep 3;26(1):40
Cellular & molecular biology letters 2021 Sep 3;26(1):40
MicroRNA-449a delays lung cancer development through inhibiting KDM3A/HIF-1α axis.
Hu S, Cao P, Kong K, Han P, Deng Y, Li F, Zhao B
Journal of translational medicine 2021 May 27;19(1):224
Journal of translational medicine 2021 May 27;19(1):224
Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice.
Liu L, Zhao Q, Kong M, Mao L, Yang Y, Xu Y
Cardiovascular research 2021 Mar 22;
Cardiovascular research 2021 Mar 22;
NG2/CSPG4, CD146/MCAM and VAP1/AOC3 are regulated by myocardin-related transcription factors in smooth muscle cells.
Rippe C, Morén B, Liu L, Stenkula KG, Mustaniemi J, Wennström M, Swärd K
Scientific reports 2021 Mar 16;11(1):5955
Scientific reports 2021 Mar 16;11(1):5955
BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level.
Kim JH, Kim J, Im SS, Lee JH, Hwang S, Chang EJ, Shin DM, Rho JK, Son J
Experimental & molecular medicine 2021 Dec;53(12):1877-1887
Experimental & molecular medicine 2021 Dec;53(12):1877-1887
BRG1 Stimulates Endothelial Derived Alarmin MRP8 to Promote Macrophage Infiltration in an Animal Model of Cardiac Hypertrophy.
Li Z, Zhang Y, Zhang Y, Yu L, Xiao B, Li T, Kong X, Xu Y
Frontiers in cell and developmental biology 2020;8:569
Frontiers in cell and developmental biology 2020;8:569
Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2.
Fan Z, Kong M, Li M, Hong W, Fan X, Xu Y
Frontiers in cell and developmental biology 2020;8:259
Frontiers in cell and developmental biology 2020;8:259
Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer.
He C, Yu L, Ding L, Yao H, Chen Y, Hao Y
Biomaterials 2020 Oct;255:120181
Biomaterials 2020 Oct;255:120181
Histone Demethylase JMJD1A Promotes Tumor Progression via Activating Snail in Prostate Cancer.
Tang DE, Dai Y, Fan LL, Geng XY, Fu DX, Jiang HW, Xu SH
Molecular cancer research : MCR 2020 May;18(5):698-708
Molecular cancer research : MCR 2020 May;18(5):698-708
Epigenetic activation of CTGF transcription by high glucose in renal tubular epithelial cells is mediated by myocardin-related transcription factor A.
Shao J, Xu H, Wu X, Xu Y
Cell and tissue research 2020 Mar;379(3):549-559
Cell and tissue research 2020 Mar;379(3):549-559
p300-Mediated Acetylation of Histone Demethylase JMJD1A Prevents Its Degradation by Ubiquitin Ligase STUB1 and Enhances Its Activity in Prostate Cancer.
Xu S, Fan L, Jeon HY, Zhang F, Cui X, Mickle MB, Peng G, Hussain A, Fazli L, Gleave ME, Dong X, Qi J
Cancer research 2020 Aug 1;80(15):3074-3087
Cancer research 2020 Aug 1;80(15):3074-3087
Histone demethylase JMJD1A promotes expression of DNA repair factors and radio-resistance of prostate cancer cells.
Fan L, Xu S, Zhang F, Cui X, Fazli L, Gleave M, Clark DJ, Yang A, Hussain A, Rassool F, Qi J
Cell death & disease 2020 Apr 1;11(4):214
Cell death & disease 2020 Apr 1;11(4):214
Epigenetic regulation of lung cancer cell proliferation and migration by the chromatin remodeling protein BRG1.
Li Z, Xia J, Fang M, Xu Y
Oncogenesis 2019 Nov 6;8(11):66
Oncogenesis 2019 Nov 6;8(11):66
Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.
Tran KA, Dillingham CM, Sridharan R
The EMBO journal 2019 Nov 15;38(22):e101681
The EMBO journal 2019 Nov 15;38(22):e101681
Acetylation of PHF5A Modulates Stress Responses and Colorectal Carcinogenesis through Alternative Splicing-Mediated Upregulation of KDM3A.
Wang Z, Yang X, Liu C, Li X, Zhang B, Wang B, Zhang Y, Song C, Zhang T, Liu M, Liu B, Ren M, Jiang H, Zou J, Liu X, Zhang H, Zhu WG, Yin Y, Zhang Z, Gu W, Luo J
Molecular cell 2019 Jun 20;74(6):1250-1263.e6
Molecular cell 2019 Jun 20;74(6):1250-1263.e6
Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.
Fan L, Zhang F, Xu S, Cui X, Hussain A, Fazli L, Gleave M, Dong X, Qi J
Proceedings of the National Academy of Sciences of the United States of America 2018 May 15;115(20):E4584-E4593
Proceedings of the National Academy of Sciences of the United States of America 2018 May 15;115(20):E4584-E4593
Epigenetic activation of PERP transcription by MKL1 contributes to ROS-induced apoptosis in skeletal muscle cells.
Li Z, Chen B, Dong W, Xu W, Song M, Fang M, Guo J, Xu Y
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Jul 27;
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Jul 27;
Impact of histone demethylase KDM3A-dependent AP-1 transactivity on hepatotumorigenesis induced by PI3K activation.
Nakatsuka T, Tateishi K, Kudo Y, Yamamoto K, Nakagawa H, Fujiwara H, Takahashi R, Miyabayashi K, Asaoka Y, Tanaka Y, Ijichi H, Hirata Y, Otsuka M, Kato M, Sakai J, Tachibana M, Aburatani H, Shinkai Y, Koike K
Oncogene 2017 Nov 9;36(45):6262-6271
Oncogene 2017 Nov 9;36(45):6262-6271
Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.
Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, Jacob C
Nature communications 2017 Jan 31;8:14272
Nature communications 2017 Jan 31;8:14272
KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.
Yeyati PL, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams IR, Playfoot C, Gilbert N, van Heyningen V, Wills J, von Kriegsheim A, Finch A, Sakai J, Schofield CJ, Jackson IJ, Mill P
The Journal of cell biology 2017 Apr 3;216(4):999-1013
The Journal of cell biology 2017 Apr 3;216(4):999-1013
JMJD1A promotes tumorigenesis and forms a feedback loop with EZH2/let-7c in NSCLC cells.
Zhan M, Wen F, Liu L, Chen Z, Wei H, Zhou H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Aug;37(8):11237-47
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Aug;37(8):11237-47
Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.
Kaukonen R, Mai A, Georgiadou M, Saari M, De Franceschi N, Betz T, Sihto H, Ventelä S, Elo L, Jokitalo E, Westermarck J, Kellokumpu-Lehtinen PL, Joensuu H, Grenman R, Ivaska J
Nature communications 2016 Aug 4;7:12237
Nature communications 2016 Aug 4;7:12237
The hypoxia-inducible epigenetic regulators Jmjd1a and G9a provide a mechanistic link between angiogenesis and tumor growth.
Ueda J, Ho JC, Lee KL, Kitajima S, Yang H, Sun W, Fukuhara N, Zaiden N, Chan SL, Tachibana M, Shinkai Y, Kato H, Poellinger L
Molecular and cellular biology 2014 Oct 1;34(19):3702-20
Molecular and cellular biology 2014 Oct 1;34(19):3702-20
A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression.
Oh HS, Bryant KF, Nieland TJ, Mazumder A, Bagul M, Bathe M, Root DE, Knipe DM
mBio 2014 Feb 4;5(1):e01086-13
mBio 2014 Feb 4;5(1):e01086-13
Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis.
Kasioulis I, Syred HM, Tate P, Finch A, Shaw J, Seawright A, Fuszard M, Botting CH, Shirran S, Adams IR, Jackson IJ, van Heyningen V, Yeyati PL
Molecular biology of the cell 2014 Apr;25(8):1216-33
Molecular biology of the cell 2014 Apr;25(8):1216-33
Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen regulates the KSHV epigenome by association with the histone demethylase KDM3A.
Kim KY, Huerta SB, Izumiya C, Wang DH, Martinez A, Shevchenko B, Kung HJ, Campbell M, Izumiya Y
Journal of virology 2013 Jun;87(12):6782-93
Journal of virology 2013 Jun;87(12):6782-93
Role of the hypoxia-related gene, JMJD1A, in hepatocellular carcinoma: clinical impact on recurrence after hepatic resection.
Yamada D, Kobayashi S, Yamamoto H, Tomimaru Y, Noda T, Uemura M, Wada H, Marubashi S, Eguchi H, Tanemura M, Doki Y, Mori M, Nagano H
Annals of surgical oncology 2012 Jul;19 Suppl 3:S355-64
Annals of surgical oncology 2012 Jul;19 Suppl 3:S355-64
The JmjC domain-containing histone demethylase KDM3A is a positive regulator of the G1/S transition in cancer cells via transcriptional regulation of the HOXA1 gene.
Cho HS, Toyokawa G, Daigo Y, Hayami S, Masuda K, Ikawa N, Yamane Y, Maejima K, Tsunoda T, Field HI, Kelly JD, Neal DE, Ponder BA, Maehara Y, Nakamura Y, Hamamoto R
International journal of cancer 2012 Aug 1;131(3):E179-89
International journal of cancer 2012 Aug 1;131(3):E179-89
Upregulation of MiR-155 in nasopharyngeal carcinoma is partly driven by LMP1 and LMP2A and downregulates a negative prognostic marker JMJD1A.
Du ZM, Hu LF, Wang HY, Yan LX, Zeng YX, Shao JY, Ernberg I
PloS one 2011 Apr 26;6(4):e19137
PloS one 2011 Apr 26;6(4):e19137
Jumonji domain containing 1A is a novel prognostic marker for colorectal cancer: in vivo identification from hypoxic tumor cells.
Uemura M, Yamamoto H, Takemasa I, Mimori K, Hemmi H, Mizushima T, Ikeda M, Sekimoto M, Matsuura N, Doki Y, Mori M
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Sep 15;16(18):4636-46
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Sep 15;16(18):4636-46
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12835-1-AP(KDM3A,JMJD1A antibody) at dilution of 1:2000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using KDM3A antibody 12835-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-KDM3A,JMJD1A (IP:12835-1-AP, 5ug; Detection:12835-1-AP 1:1000) with HeLa cells lysate 2500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary tumor using 12835-1-AP(KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue