12835-1-AP

antibody from Proteintech Group

Targeting: KDM3A JHMD2A, JMJD1, JMJD1A, KIAA0742, TSGA

Provider product page for 12835-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [41]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 12835-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12835-1-AP, RRID:AB_1072935
Product name: KDM3A,JMJD1A antibody
Antibody type: Polyclonal
Description: KD/KO validated KDM3A,JMJD1A antibody (Cat. #12835-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

KDM3A protein structure - 12835-1-AP shown in red.

Submitted references KDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing.

Dillingham CM, Cormaty H, Morgan EC, Tak AI, Esgdaille DE, Boutz PL, Sridharan R
bioRxiv : the preprint server for biology 2024 Jan 23;

Lysine demethylase KDM3A alleviates hyperoxia-induced bronchopulmonary dysplasia in mice by promoting ETS1 expression.

Yang M, Chen Y, Huang X, Shen F, Meng Y
Experimental cell research 2024 Feb 15;435(2):113945

Glutamine-mediated epigenetic regulation of cFLIP underlies resistance to TRAIL in pancreatic cancer.

Kim JH, Lee J, Im SS, Kim B, Kim EY, Min HJ, Heo J, Chang EJ, Choi KC, Shin DM, Son J
Experimental & molecular medicine 2024 Apr;56(4):1013-1026

The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis.

Wang Z, Li T, Liu D, Li M, Liu S, Yu X, Li H, Song H, Zhao W, Liu Z, Chen X, Lu G, Chen ZJ, Huang T, Liu H
iScience 2024 Apr 19;27(4):109456

Suppression of breast cancer-associated bone loss with osteoblast proteomes via Hsp90ab1/moesin-mediated inhibition of TGFβ/FN1/CD44 signaling.

Sun X, Li K, Hase M, Zha R, Feng Y, Li BY, Yokota H
Theranostics 2022;12(2):929-943

KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development.

Wang W, Wang B
BMC cancer 2022 May 19;22(1):562

Liu L, Zhao Q, Kong M, Mao L, Yang Y, Xu Y
Cardiovascular research 2022 Feb 21;118(3):844-858

Epigenetic drug screen identified IOX1 as an inhibitor of Th17-mediated inflammation through targeting TET2.

Hu X, Zou Y, Copland DA, Schewitz-Bowers LP, Li Y, Lait PJP, Stimpson M, Zhang Z, Guo S, Liang J, Chen T, Li JJ, Yuan S, Li S, Zhou P, Liu Y, Dick AD, Wen X, Lee RWJ, Wei L
EBioMedicine 2022 Dec;86:104333

MiR-22-3p regulates the proliferation, migration and invasion of colorectal cancer cells by directly targeting KDM3A through the Hippo pathway.

Jin RR, Zeng C, Chen Y
Histology and histopathology 2022 Dec;37(12):1241-1252

JMJD1A/NR4A1 Signaling Regulates the Procession of Renal Tubular Epithelial Interstitial Fibrosis Induced by AGEs in HK-2.

Wang S, Zuo A, Jiang W, Xie J, Lin H, Sun W, Zhao M, Xia J, Shao J, Zhao X, Liang D, Yang A, Sun J, Wang M
Frontiers in medicine 2021;8:807694

PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.

Liu W, Wang C, Wang S, Zeng K, Wei S, Sun N, Sun G, Wang M, Zou R, Liu W, Lin L, Song H, Jin Z, Zhao Y
International journal of biological sciences 2021;17(1):188-203

HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury.

Zhao M, Wang S, Zuo A, Zhang J, Wen W, Jiang W, Chen H, Liang D, Sun J, Wang M
Cellular & molecular biology letters 2021 Sep 3;26(1):40

MicroRNA-449a delays lung cancer development through inhibiting KDM3A/HIF-1α axis.

Hu S, Cao P, Kong K, Han P, Deng Y, Li F, Zhao B
Journal of translational medicine 2021 May 27;19(1):224

NG2/CSPG4, CD146/MCAM and VAP1/AOC3 are regulated by myocardin-related transcription factors in smooth muscle cells.

Rippe C, Morén B, Liu L, Stenkula KG, Mustaniemi J, Wennström M, Swärd K
Scientific reports 2021 Mar 16;11(1):5955

BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level.

Kim JH, Kim J, Im SS, Lee JH, Hwang S, Chang EJ, Shin DM, Rho JK, Son J
Experimental & molecular medicine 2021 Dec;53(12):1877-1887

BRG1 Stimulates Endothelial Derived Alarmin MRP8 to Promote Macrophage Infiltration in an Animal Model of Cardiac Hypertrophy.

Li Z, Zhang Y, Zhang Y, Yu L, Xiao B, Li T, Kong X, Xu Y
Frontiers in cell and developmental biology 2020;8:569

Fan Z, Kong M, Li M, Hong W, Fan X, Xu Y
Frontiers in cell and developmental biology 2020;8:259

Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer.

He C, Yu L, Ding L, Yao H, Chen Y, Hao Y
Biomaterials 2020 Oct;255:120181

Histone Demethylase JMJD1A Promotes Tumor Progression via Activating Snail in Prostate Cancer.

Tang DE, Dai Y, Xu SH
Molecular cancer research : MCR 2020 May;18(5):698-708

Epigenetic activation of CTGF transcription by high glucose in renal tubular epithelial cells is mediated by myocardin-related transcription factor A.

Shao J, Xu H, Wu X, Xu Y
Cell and tissue research 2020 Mar;379(3):549-559

p300-Mediated Acetylation of Histone Demethylase JMJD1A Prevents Its Degradation by Ubiquitin Ligase STUB1 and Enhances Its Activity in Prostate Cancer.

Xu S, Fan L, Jeon HY, Zhang F, Cui X, Mickle MB, Peng G, Hussain A, Fazli L, Gleave ME, Dong X, Qi J
Cancer research 2020 Aug 1;80(15):3074-3087

Histone demethylase JMJD1A promotes expression of DNA repair factors and radio-resistance of prostate cancer cells.

Fan L, Xu S, Zhang F, Cui X, Fazli L, Gleave M, Clark DJ, Yang A, Hussain A, Rassool F, Qi J
Cell death & disease 2020 Apr 1;11(4):214

Epigenetic regulation of lung cancer cell proliferation and migration by the chromatin remodeling protein BRG1.

Li Z, Xia J, Fang M, Xu Y
Oncogenesis 2019 Nov 6;8(11):66

KDM3A and KDM4C Regulate Mesenchymal Stromal Cell Senescence and Bone Aging via Condensin-mediated Heterochromatin Reorganization.

Huang B, Wang B, Yuk-Wai Lee W, Pong U K, Leung KT, Li X, Liu Z, Chen R, Lin JC, Tsang LL, Liu B, Ruan YC, Chan HC, Li G, Jiang X
iScience 2019 Nov 22;21:375-390

Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.

Tran KA, Dillingham CM, Sridharan R
The EMBO journal 2019 Nov 15;38(22):e101681

Acetylation of PHF5A Modulates Stress Responses and Colorectal Carcinogenesis through Alternative Splicing-Mediated Upregulation of KDM3A.

Wang Z, Yang X, Liu C, Li X, Zhang B, Wang B, Zhang Y, Song C, Zhang T, Liu M, Liu B, Ren M, Jiang H, Zou J, Liu X, Zhang H, Zhu WG, Yin Y, Zhang Z, Gu W, Luo J
Molecular cell 2019 Jun 20;74(6):1250-1263.e6

Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.

Fan L, Zhang F, Xu S, Cui X, Hussain A, Fazli L, Gleave M, Dong X, Qi J
Proceedings of the National Academy of Sciences of the United States of America 2018 May 15;115(20):E4584-E4593

Epigenetic activation of PERP transcription by MKL1 contributes to ROS-induced apoptosis in skeletal muscle cells.

Li Z, Chen B, Dong W, Xu W, Song M, Fang M, Guo J, Xu Y
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Jul 27;

Impact of histone demethylase KDM3A-dependent AP-1 transactivity on hepatotumorigenesis induced by PI3K activation.

Nakatsuka T, Tateishi K, Kudo Y, Yamamoto K, Nakagawa H, Fujiwara H, Takahashi R, Miyabayashi K, Asaoka Y, Tanaka Y, Ijichi H, Hirata Y, Otsuka M, Kato M, Sakai J, Tachibana M, Aburatani H, Shinkai Y, Koike K
Oncogene 2017 Nov 9;36(45):6262-6271

Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.

Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, Jacob C
Nature communications 2017 Jan 31;8:14272

KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.

Yeyati PL, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams IR, Playfoot C, Gilbert N, van Heyningen V, Wills J, von Kriegsheim A, Finch A, Sakai J, Schofield CJ, Jackson IJ, Mill P
The Journal of cell biology 2017 Apr 3;216(4):999-1013

JMJD1A promotes tumorigenesis and forms a feedback loop with EZH2/let-7c in NSCLC cells.

Zhan M, Wen F, Liu L, Chen Z, Wei H, Zhou H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Aug;37(8):11237-47

Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.

Kaukonen R, Mai A, Georgiadou M, Saari M, De Franceschi N, Betz T, Sihto H, Ventelä S, Elo L, Jokitalo E, Westermarck J, Kellokumpu-Lehtinen PL, Joensuu H, Grenman R, Ivaska J
Nature communications 2016 Aug 4;7:12237

The hypoxia-inducible epigenetic regulators Jmjd1a and G9a provide a mechanistic link between angiogenesis and tumor growth.

Ueda J, Ho JC, Lee KL, Kitajima S, Yang H, Sun W, Fukuhara N, Zaiden N, Chan SL, Tachibana M, Shinkai Y, Kato H, Poellinger L
Molecular and cellular biology 2014 Oct 1;34(19):3702-20

A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression.

Oh HS, Bryant KF, Nieland TJ, Mazumder A, Bagul M, Bathe M, Root DE, Knipe DM
mBio 2014 Feb 4;5(1):e01086-13

Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis.

Kasioulis I, Syred HM, Tate P, Finch A, Shaw J, Seawright A, Fuszard M, Botting CH, Shirran S, Adams IR, Jackson IJ, van Heyningen V, Yeyati PL
Molecular biology of the cell 2014 Apr;25(8):1216-33

Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen regulates the KSHV epigenome by association with the histone demethylase KDM3A.

Kim KY, Huerta SB, Izumiya C, Wang DH, Martinez A, Shevchenko B, Kung HJ, Campbell M, Izumiya Y
Journal of virology 2013 Jun;87(12):6782-93

Role of the hypoxia-related gene, JMJD1A, in hepatocellular carcinoma: clinical impact on recurrence after hepatic resection.

Yamada D, Kobayashi S, Yamamoto H, Tomimaru Y, Noda T, Uemura M, Wada H, Marubashi S, Eguchi H, Tanemura M, Doki Y, Mori M, Nagano H
Annals of surgical oncology 2012 Jul;19 Suppl 3:S355-64

The JmjC domain-containing histone demethylase KDM3A is a positive regulator of the G1/S transition in cancer cells via transcriptional regulation of the HOXA1 gene.

Cho HS, Toyokawa G, Daigo Y, Hayami S, Masuda K, Ikawa N, Yamane Y, Maejima K, Tsunoda T, Field HI, Kelly JD, Neal DE, Ponder BA, Maehara Y, Nakamura Y, Hamamoto R
International journal of cancer 2012 Aug 1;131(3):E179-89

Upregulation of MiR-155 in nasopharyngeal carcinoma is partly driven by LMP1 and LMP2A and downregulates a negative prognostic marker JMJD1A.

Du ZM, Hu LF, Wang HY, Yan LX, Zeng YX, Shao JY, Ernberg I
PloS one 2011 Apr 26;6(4):e19137

Jumonji domain containing 1A is a novel prognostic marker for colorectal cancer: in vivo identification from hypoxic tumor cells.

Uemura M, Yamamoto H, Takemasa I, Mimori K, Hemmi H, Mizushima T, Ikeda M, Sekimoto M, Matsuura N, Doki Y, Mori M
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Sep 15;16(18):4636-46

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 12835-1-AP(KDM3A,JMJD1A antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using KDM3A antibody 12835-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-KDM3A,JMJD1A (IP:12835-1-AP, 5ug; Detection:12835-1-AP 1:1000) with HeLa cells lysate 2500ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary tumor using 12835-1-AP(KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue