AF6746

antibody from R&D Systems

Targeting: KDM3A JHMD2A, JMJD1, JMJD1A, KIAA0742, TSGA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6746

Provider

R&D Systems

Product name

Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human KDM3A in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reactivity with recombinant human (rh) KDM4A is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9Y4C1


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human KDM3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6746) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KDM3A at approximately 150 kDa (as indicated), the band at approximately 70kDa represents the product of degradation or processing. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.