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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- A-718 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SUMO2/3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody detects endogenous, human SUMOylated proteins in Western blots. This antibody has equivalent reactivity to SUMO2 and SUMO3 in Western blots with recombinant SUMO proteins. It has less than 5% cross-reactivity with recombinant SUMO4 and no cross-reactivity with recombinant SUMO1.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P55854- Isotype
- IgG
- Antibody clone number
- 852908
- Vial size
- 100 ug
- Storage
- Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SUMO2/3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO2/3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SUMO2/3 by Western Blot. Western blot shows lysates of SUMO2/3. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO2/3 at approximately 16-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
