Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- R33164 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Pericentrin 1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific Pericentrin 1 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the Pericentrin 1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa lysate with Pericentrin 1 antibody at 1ug/ml. Predicted molecular weight: ~75kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa lysate with Pericentrin 1 antibody at 1ug/ml. Predicted molecular weight: ~75kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human HeLa, B) mouse NIH 3T3, C) human HeLa (nuclear fraction) and D) human HEK293 (nuclear fraction) cell lysate with Pericentrin 1 antibody at 0.5ug/ml. Predicted molecular weight: ~75 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human U-2 OS cells with Pericentrin 1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human MCF7 cells with Pericentrin 1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human MCF7 cells with Pericentrin 1 antibody (blue) at 10ug/ml and naive goat Ig (black).