PA5-34387

antibody from Invitrogen Antibodies

Targeting: SDHD cybS, PGL, PGL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34387

Provider

Invitrogen Antibodies

Product name

SDHD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is EL4 cell lysate. PA5-34387 can be used with blocking peptide PEP-1430.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Bcl2l10 induces metabolic alterations in ovarian cancer cells by regulating the TCA cycle enzymes SDHD and IDH1.

Lee SY, Kwon J, Lee KA
Oncology reports 2021 Apr;45(4)

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High fat, high sucrose diet causes cardiac mitochondrial dysfunction due in part to oxidative post-translational modification of mitochondrial complex II.

Sverdlov AL, Elezaby A, Behring JB, Bachschmid MM, Luptak I, Tu VH, Siwik DA, Miller EJ, Liesa M, Shirihai OS, Pimentel DR, Cohen RA, Colucci WS
Journal of molecular and cellular cardiology 2015 Jan;78:165-73

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EL4 cell lysate using a SDHD polyclonal antibody (Product # PA5-34387) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SDHD in EL4 cell lysate with SDHD Polyclonal Antibody (Product # PA5-34387) at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EL4 cells using a SDHD polyclonal antibody (Product # PA5-34387) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry staining of EL4 cells using a SDHD polyclonal antibody (Product # PA5-34387) at a 25 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of SDHD in EL4 cells with SDHD Polyclonal Antibody (Product # PA5-34387) at 2.5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of SDHD in EL4 cells with SDHD Polyclonal Antibody (Product # PA5-34387) at 2.5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of SDHD in EL4 cells with SDHD Polyclonal Antibody (Product # PA5-34387) at 20 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Bcl2l10-knockdown significantly decreases the protein expression levels of SDHD and IDH1. (A) SKOV3 and A2780 cells were transfected with siNC or siBcl2l10 and harvested 48 h after transfection. The protein expression levels of BCL2L10, SDHD, IDH1 and FH were measured by western blot analysis using beta-actin as an internal control gene. (B) Protein signals of BCL2L10, SDHD, IDH1 and FH were measured and normalized to the corresponding beta-actin signals. The relative expression levels of proteins were calculated by comparing all the normalized signals to that of the negative control group. All the experiments were repeated at least three times, and the data are expressed as the mean +- SEM. *P