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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [5]
- Other assay [1]
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- Product number
- PA5-34387 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SDHD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is EL4 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Bcl2l10 induces metabolic alterations in ovarian cancer cells by regulating the TCA cycle enzymes SDHD and IDH1.
High fat, high sucrose diet causes cardiac mitochondrial dysfunction due in part to oxidative post-translational modification of mitochondrial complex II.
Lee SY, Kwon J, Lee KA
Oncology reports 2021 Apr;45(4)
Oncology reports 2021 Apr;45(4)
High fat, high sucrose diet causes cardiac mitochondrial dysfunction due in part to oxidative post-translational modification of mitochondrial complex II.
Sverdlov AL, Elezaby A, Behring JB, Bachschmid MM, Luptak I, Tu VH, Siwik DA, Miller EJ, Liesa M, Shirihai OS, Pimentel DR, Cohen RA, Colucci WS
Journal of molecular and cellular cardiology 2015 Jan;78:165-73
Journal of molecular and cellular cardiology 2015 Jan;78:165-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EL4 cell lysate using a SDHD polyclonal antibody (Product # PA5-34387) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SDHD in EL4 cell lysate with SDHD Polyclonal Antibody (Product # PA5-34387) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EL4 cells using a SDHD polyclonal antibody (Product # PA5-34387) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of EL4 cells using a SDHD polyclonal antibody (Product # PA5-34387) at a 25 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of SDHD in EL4 cells with SDHD Polyclonal Antibody (Product # PA5-34387) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of SDHD in EL4 cells with SDHD Polyclonal Antibody (Product # PA5-34387) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of SDHD in EL4 cells with SDHD Polyclonal Antibody (Product # PA5-34387) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Bcl2l10-knockdown significantly decreases the protein expression levels of SDHD and IDH1. (A) SKOV3 and A2780 cells were transfected with siNC or siBcl2l10 and harvested 48 h after transfection. The protein expression levels of BCL2L10, SDHD, IDH1 and FH were measured by western blot analysis using beta-actin as an internal control gene. (B) Protein signals of BCL2L10, SDHD, IDH1 and FH were measured and normalized to the corresponding beta-actin signals. The relative expression levels of proteins were calculated by comparing all the normalized signals to that of the negative control group. All the experiments were repeated at least three times, and the data are expressed as the mean +- SEM. *P
