Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 21193-1-AP - Provider product page

- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#21193-1-AP, RRID:AB_10733640
- Product name
- GOLGA3 antibody
- Antibody type
- Polyclonal
- Description
- GOLGA3 antibody (Cat. #21193-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Multiple golgins are required to support extracellular matrix secretion, modification, and assembly.
TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy.
Molecular Insights into the Regulation of GNPTAB by TMEM251.
Morphological determination of localization and function of Golgi proteins.
Common Markers and Small Molecule Inhibitors in Golgi Studies.
Immunofluorescence Microscopy of the Mammalian Golgi Apparatus.
Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis.
GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway.
GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.
A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.
Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.
The mechanism on phosphorylation of Hsp20Ser16 inhibit GA stress and ER stress during OGD/R.
SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly.
Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.
Thompson G, Hoyle A, Lewis PA, Prada-Sanchez ME, Swift J, Heesom K, Lowe M, Stephens D, Stevenson NL
The Journal of cell biology 2025 Oct 6;224(10)
The Journal of cell biology 2025 Oct 6;224(10)
TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy.
Yang J, Dong Y, Xu J, Qian X, Cai Y, Chen Y, Zhang P, Gao D, Cui Z, Cui Y
Developmental cell 2025 Nov 3;60(21):2862-2879.e8
Developmental cell 2025 Nov 3;60(21):2862-2879.e8
Molecular Insights into the Regulation of GNPTAB by TMEM251.
Yang X, Doray B, Venkatarangan V, Jennings BC, Henn D, Liang J, Zhao H, Zhang W, Zhang B, Yu L, Chen L, Kornfeld S, Li M
bioRxiv : the preprint server for biology 2024 Dec 5;
bioRxiv : the preprint server for biology 2024 Dec 5;
Morphological determination of localization and function of Golgi proteins.
Xing Y, Jian Y, Zhao X, Zhang Y, Zhang Z, Zhang X, Zhang X
Biophysics reports 2024 Apr 30;10(2):121-132
Biophysics reports 2024 Apr 30;10(2):121-132
Common Markers and Small Molecule Inhibitors in Golgi Studies.
Bui S, Stark D, Li J, Zhang J, Wang Y
Methods in molecular biology (Clifton, N.J.) 2023;2557:453-493
Methods in molecular biology (Clifton, N.J.) 2023;2557:453-493
Immunofluorescence Microscopy of the Mammalian Golgi Apparatus.
Arab M, Nayak SC, Vitali T, Lowe M
Methods in molecular biology (Clifton, N.J.) 2023;2557:101-111
Methods in molecular biology (Clifton, N.J.) 2023;2557:101-111
Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis.
Xu C, Zhu M, Zhao S, Zhang X, Wang Y, Liu M
PeerJ 2023;11:e15133
PeerJ 2023;11:e15133
GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway.
Zhang W, Yang X, Li Y, Yu L, Zhang B, Zhang J, Cho WJ, Venkatarangan V, Chen L, Burugula BB, Bui S, Wang Y, Duan C, Kitzman JO, Li M
Nature communications 2022 Sep 12;13(1):5351
Nature communications 2022 Sep 12;13(1):5351
GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.
Ahat E, Bui S, Zhang J, da Veiga Leprevost F, Sharkey L, Reid W, Nesvizhskii AI, Paulson HL, Wang Y
The Journal of biological chemistry 2022 Aug;298(8):102219
The Journal of biological chemistry 2022 Aug;298(8):102219
A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.
Zhang W, Yang X, Chen L, Liu YY, Venkatarangan V, Reist L, Hanson P, Xu H, Wang Y, Li M
PLoS biology 2021 Jul;19(7):e3001361
PLoS biology 2021 Jul;19(7):e3001361
Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.
Ireland SC, Huang H, Zhang J, Li J, Wang Y
Molecular biology of the cell 2020 Aug 1;31(17):1931-1942
Molecular biology of the cell 2020 Aug 1;31(17):1931-1942
The mechanism on phosphorylation of Hsp20Ser16 inhibit GA stress and ER stress during OGD/R.
Lu T, Zou Y, Zhou X, Peng W, Hu Z
PloS one 2019;14(3):e0213410
PloS one 2019;14(3):e0213410
SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly.
Zhang X, Brachner A, Kukolj E, Slade D, Wang Y
Journal of cell science 2019 Nov 1;132(21)
Journal of cell science 2019 Nov 1;132(21)
Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.
Joshi G, Chi Y, Huang Z, Wang Y
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 1;111(13):E1230-9
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 1;111(13):E1230-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP(GOLGA3 antibody) at dilution of 1:2000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunofluorescent analysis of HepG2 cells, using GOLGA3 antibody 21193-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line