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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP1812C - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1812c, RRID:AB_10258043
- Product name
- ATG5 Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant Protein
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.78 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function.
Jaber N, Dou Z, Chen JS, Catanzaro J, Jiang YP, Ballou LM, Selinger E, Ouyang X, Lin RZ, Zhang J, Zong WX
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 7;109(6):2003-8
Proceedings of the National Academy of Sciences of the United States of America 2012 Feb 7;109(6):2003-8
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- APG5 Antibody (Cat.#AP1812c) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the APG5 antibody detected the APG5 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cells stained with APG5 antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812c APG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells."
- Primary Ab dilution
- 1:200
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- APG5 Antibody (Cat. #AP1812cb)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG5 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
