AM20205PU-N

antibody from Acris Antibodies GmbH

Targeting: ATG5 APG5, APG5L, ASP, hAPG5

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20205PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20205PU-N, RRID:AB_10754131

Product name

anti APG5L / ATG5 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Recombinant ATG5

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

7C6

Vial size

0.1 mg

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Monoclonal antibody ATG5-7C6 detects the endogenous Atg5 as well as the overexpressed 33 kDa-protein in Jurkat cells. Cells were fixed with 4% paraformaldehyd for 5 min and permeabilized with 0.05% Saponin. After 10 min aceton-treatment, Atg5 was detected with mab ATG5-7C6 at 0.5 µg/ml. Images by courtesy of Hans-Uwe Simon, MD, PhD, University of Bern.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence staining and Analysis by Flow Cytometry: AM20205PU-N ATG5 antibody detects the endogenous Atg5 as well as the overexpressed 33 kDa protein in Jurkat cells.