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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F46218 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ATG5 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ATG5 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the ATG5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of ATG5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- APG5 antibody western blot analysis in HeLa lysate. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from SH-SY5Y cell line using APG5/ ATG5 antibody at 1:1000. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of U251 cells stained with ATG5 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with the ATG5 antibody
