PAB0712

antibody from Abnova Corporation

Targeting: ATG5 APG5, APG5L, ASP, hAPG5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0712

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0712, RRID:AB_1112875

Product name

ATG5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ATG5.

Storage

Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains.

Itoh H, Matsuo H, Kitamura N, Yamamoto S, Higuchi T, Takematsu H, Kamikubo Y, Kondo T, Yamashita K, Sasada M, Takaori-Kondo A, Adachi S
Journal of leukocyte biology 2015 Jul;98(1):107-17

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot on non - reduced human neutrophils using rabbit ATG5 polyclonal antibody (Cat # PAB0712) at a dilution of 1 : 100 . The detected band has an apparent molecular weight of 49 KDa corresponding to the ATG5-ATG12 complex molecule.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Confocal microscopy on stained ATG5 using rabbit ATG5 polyclonal antibody (Cat # PAB0712) in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) . ATG5 appears green. Red staining is p25.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DAB staining of ATG5 in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) using rabbit ATG5 polyclonal antibody (Cat # PAB0712) .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)