Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB0712 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0712, RRID:AB_1112875
- Product name
- ATG5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains.
Itoh H, Matsuo H, Kitamura N, Yamamoto S, Higuchi T, Takematsu H, Kamikubo Y, Kondo T, Yamashita K, Sasada M, Takaori-Kondo A, Adachi S
Journal of leukocyte biology 2015 Jul;98(1):107-17
Journal of leukocyte biology 2015 Jul;98(1):107-17
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot on non - reduced human neutrophils using rabbit ATG5 polyclonal antibody (Cat # PAB0712) at a dilution of 1 : 100 . The detected band has an apparent molecular weight of 49 KDa corresponding to the ATG5-ATG12 complex molecule.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on stained ATG5 using rabbit ATG5 polyclonal antibody (Cat # PAB0712) in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) . ATG5 appears green. Red staining is p25.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DAB staining of ATG5 in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) using rabbit ATG5 polyclonal antibody (Cat # PAB0712) .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)