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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB12482 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12482, RRID:AB_10550514
- Product name
- ATG5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
- Storage
- Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells.
Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus.
Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Cadwell K, Liu JY, Brown SL, Miyoshi H, Loh J, Lennerz JK, Kishi C, Kc W, Carrero JA, Hunt S, Stone CD, Brunt EM, Xavier RJ, Sleckman BP, Li E, Mizushima N, Stappenbeck TS, Virgin HW 4th
Nature 2008 Nov 13;456(7219):259-63
Nature 2008 Nov 13;456(7219):259-63
Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus.
Wang Y, Singh R, Massey AC, Kane SS, Kaushik S, Grant T, Xiang Y, Cuervo AM, Czaja MJ
The Journal of biological chemistry 2008 Feb 22;283(8):4766-77
The Journal of biological chemistry 2008 Feb 22;283(8):4766-77
Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Pyo JO, Nah J, Kim HJ, Lee HJ, Heo J, Lee H, Jung YK
Autophagy 2008 Apr;4(3):315-21
Autophagy 2008 Apr;4(3):315-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) using ATG5 polyclonal antibody (Cat # PAB12482). Lane 2 : ATG5 KO ES cell lysate (negative control). Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ATG5 on SH-SY5Y cells using ATG5 polyclonal antibody (Cat # PAB12482) at 1 : 250. Photo courtesy of an anonymous collaborator.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Staining of ATG5 in human liver hepatocytes using ATG5 polyclonal antibody (Cat # PAB12482) at 2.5 ug/mL .
- Validation comment
- Immunohistochemistry
