V3951

antibody from NSJ Bioreagents

Targeting: ATG5 APG5, APG5L, ASP, hAPG5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3951

Provider

NSJ Bioreagents

Product name

ATG5 Antibody

Antibody type

Monoclonal

Description

This highly specific ATG5 antibody is suitable for use in ELISA/Western blot/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

ATG5/2101

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the ATG5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human THP1 and human brain lysate with ATG5 antibody (clone ATG5/2101). Expected molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human endometrium with ATG5 antibody (clone ATG5/2101). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain with ATG5 antibody (clone ATG5/2101). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ATG5 antibody (clone ATG5/2101). These results demonstrate the foremost specificity of the ATG5/2101 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free ATG5 antibody (clone ATG5/2101) as confirmation of integrity and purity.