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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- HPA042973 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA042973, RRID:AB_2678246
- Product name
- Anti-ATG5
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ATG5, Gene description: autophagy related 5, Alternative Gene Names: APG5, APG5L, ASP, hAPG5, Validated applications: WB, IHC, ICC, Uniprot ID: Q9H1Y0, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Evidence for the involvement of lipid rafts localized at the ER-mitochondria associated membranes in autophagosome formation
Garofalo T, Matarrese P, Manganelli V, Marconi M, Tinari A, Gambardella L, Faggioni A, Misasi R, Sorice M, Malorni W
Autophagy 2016;12(6):917-935
Autophagy 2016;12(6):917-935
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and ATG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401532).
- Sample type
- Human
- Protocol
- Protocol
