Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- ABIN258388 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (AA 1-50), (N-Term) antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine, Simian, Xenopus, Zebrafish
- Host
- Rabbit
- Epitope
- N-Term, AA 1-50
- Vial size
- 0.025 mL
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
Submitted references Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus.
Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Wang Y, Singh R, Massey AC, Kane SS, Kaushik S, Grant T, Xiang Y, Cuervo AM, Czaja MJ
The Journal of biological chemistry 2008 Feb 22;283(8):4766-77
The Journal of biological chemistry 2008 Feb 22;283(8):4766-77
Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Pyo JO, Nah J, Kim HJ, Lee HJ, Heo J, Lee H, Jung YK
Autophagy 2008 Apr;4(3):315-21
Autophagy 2008 Apr;4(3):315-21
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Supportive validation
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- WB
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- WB
Supportive validation
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- Experimental details
- Immunofluorescent staining of SY5Y cells using ABIN258387 at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.
Supportive validation
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- IHC
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- IHC
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- IHC