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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00055743-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055743-M01, RRID:AB_426020
- Product name
- CHFR monoclonal antibody (M01), clone 1H3-A12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CHFR.
- Antigen sequence
MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRK
REWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSG
QVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYL
VYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAG
AGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPT
ASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSV
ASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVK
KKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAA
GKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAA
CYSGWMERSSLCPTCRCPVERICKNHILNNLVEAY
LIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRS
FSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCP
EYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSL
TTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQD
PRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFC
ELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWK
NMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLR
SFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCR
TQVKAHHAMKFNHICEQTRFKN- Isotype
- IgG
- Antibody clone number
- 1H3-A12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach.
CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer.
Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.
Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.
Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.
Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression.
Fujita A, Yamamoto H, Imamura M, Nakamura N, Maehara Y, Tsuneyoshi M, Oda Y
Virchows Archiv : an international journal of pathology 2012 Feb;460(2):163-9
Virchows Archiv : an international journal of pathology 2012 Feb;460(2):163-9
CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T
The Journal of biological chemistry 2012 Apr 13;287(16):12975-84
The Journal of biological chemistry 2012 Apr 13;287(16):12975-84
Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer.
Hu SL, Huang DB, Sun YB, Wu L, Xu WP, Yin S, Chen J, Jiang XD, Shen G
Medical oncology (Northwood, London, England) 2011 Jun;28(2):447-54
Medical oncology (Northwood, London, England) 2011 Jun;28(2):447-54
Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.
Privette LM, Weier JF, Nguyen HN, Yu X, Petty EM
Neoplasia (New York, N.Y.) 2008 Jul;10(7):643-52
Neoplasia (New York, N.Y.) 2008 Jul;10(7):643-52
Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.
Gao YJ, Xin Y, Zhang JJ, Zhou J
World journal of gastroenterology 2008 Aug 28;14(32):5000-7
World journal of gastroenterology 2008 Aug 28;14(32):5000-7
Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.
Milne AN, Sitarz R, Carvalho R, Polak MM, Ligtenberg M, Pauwels P, Offerhaus GJ, Weterman MA
Human pathology 2007 Jun;38(6):903-13
Human pathology 2007 Jun;38(6):903-13
Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression.
Privette LM, González ME, Ding L, Kleer CG, Petty EM
Cancer research 2007 Jul 1;67(13):6064-74
Cancer research 2007 Jul 1;67(13):6064-74
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CHFR monoclonal antibody (M01), clone 1H3-A12. Western Blot analysis of CHFR expression in 293.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
