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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- CF504443 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIF4GD Monoclonal Antibody (OTI4C11), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4C11
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIF4GD (RC207489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MIF4G. Positive lysates LY402799 (100 µg) and LC402799 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-MIF4GD mouse monoclonal antibody (TA504443) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIF4GD(RC207489).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-MIF4GD mouse monoclonal antibody (TA504443).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207489 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIF4GD antibody (TA504443), and then analyzed by flow cytometry.
