- UM500057 - OriGene SDCBP antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SDCBP monoclonal antibody (Clone UMAB69).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SDCBP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of MCF-7 cells using anti-SDCBP mouse monoclonal antibody (UM500057, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SDCBP mouse monoclonal antibody. (UM500057; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SDCBP mouse monoclonal antibody. (UM500057; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded composit of 3 human liver cancer cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded composit of 3 human endometrial cancer cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded composit of 3 human ovarian cancer cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded composit of 2 human spleen cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in the cells of red pulp and white pulp.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded tonsil using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in germinal and non germinal center of the the tonsil .
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded composit of 2 human melanoma cases using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane, nuclear, and cytoplasmic staining is seen in tumor cells with various intensity.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human brain using anti-SDCBP clone UMAB69 mouse monoclonal antibody (UM500057) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Weak membrane and cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SDCBP mouse monoclonal antibody (UM500057). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Validation comment: 10K-CHIP

UM500057