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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-28813 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntenin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: U87-MG. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Manganese systemic distribution is modulated in vivo during tumor progression and affects tumor cell migration and invasion in vitro.
Stelling MP, Soares MA, Cardoso SC, Motta JM, de Abreu JC, Antunes MJM, de Freitas VG, Moraes JA, Castelo-Branco MTL, Pérez CA, Pavão MSG
Scientific reports 2021 Aug 4;11(1):15833
Scientific reports 2021 Aug 4;11(1):15833
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Syntenin 1 in paraformaldehyde-fixed HeLa cells using a Syntenin 1 polyclonal antibody (Product # PA5-28813) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Syntenin 1 was performed in U87-MG whole cell extracts using 5 µg of Syntenin 1 Polyclonal Antibody (Product # PA5-28813). Samples were transferred to a membrane and probed with Syntenin 1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Tumor cell-derived extracellular vesicles are enriched in manganese and affect tumor cell migration. LLC cells-derived extracellular vesicles were concentrated by ultracentrifugation. ( a ) EVs quantification and size determination. N = 6. ( b ) CD63 and syntenin-1 detection by Western blotting. Ponceau was used as total protein control. ( c ) Tumor cells, EV-enriched fraction, EV-free conditioned medium and basal medium were analyzed by X-Ray Fluorescence. N = 6. Units are expressed in concentration as ppm (parts per million). Control cells--white bars; Mn-pretreated cells (Mn) - black bars. *p < 0.05, Student's T test. ( d ) Wound healing assays of LLC cells incubated with extracellular vesicle-enriched medium from control and Mn-pretreated LLC cells. N = 12. *p < 0.05; **p < 0.01, one-way ANOVA and Bonferroni's Multiple Comparison Test.
