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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-21562 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INPP5F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse brain. Predicted reactivity: Mouse (91%), Rat (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INPP5F using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an INPP5F polyclonal antibody (Product # PA5-21562) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INPP5F using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an INPP5F polyclonal antibody (Product # PA5-21562) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INPP5F was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a INPP5F Polyclonal Antibody (Product # PA5-21562) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of INPP5F was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: INPP5F Polyclonal Antibody (Product # PA5-21562) diluted at 1:500. Blue: Hoechst 33342 staining.
