17797-1-AP

antibody from Proteintech Group

Targeting: INPP5E CORS1, JBTS1, pharbin, PPI5PIV

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17797-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#17797-1-AP, RRID:AB_2167120

Product name

INPP5E antibody

Antibody type

Polyclonal

Description

KD/KO validated INPP5E antibody (Cat. #17797-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure.

Wang X, Yu J, Yue H, Li S, Yang A, Zhu Z, Guan Z, Wang J
Molecular neurobiology 2024 Sep;61(9):6189-6199

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XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis.

Hong R, Tan Y, Tian X, Huang Z, Wang J, Ni H, Yang J, Bu W, Yang S, Li T, Yu F, Zhong W, Sun T, Wang X, Li D, Liu M, Yang Y, Zhou J
EMBO reports 2024 Mar;25(3):1055-1074

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Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging.

Schueder F, Rivera-Molina F, Su M, Marin Z, Kidd P, Rothman JE, Toomre D, Bewersdorf J
Cell 2024 Mar 28;187(7):1769-1784.e18

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High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies.

Hosoba K, Morita T, Zhang Y, Kishi H, Yamamoto T, Miyamoto T
Methods in cell biology 2023;175:85-95

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INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control.

Chiu TY, Lo CH, Lin YH, Lai YD, Lin SS, Fang YT, Huang WS, Huang SY, Tsai PY, Yang FH, Chong WM, Wu YC, Tsai HC, Liu YW, Hsu CL, Liao JC, Wang WJ
Communications biology 2023 Sep 5;6(1):911

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Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia.

Shi P, Tian J, Mallinger JC, Ling D, Deleyrolle LP, McIntyre JC, Caspary T, Breunig JJ, Sarkisian MR
Cells 2023 Sep 26;12(19)

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Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia.

Palicharla VR, Hwang SH, Somatilaka BN, Legué E, Shimada IS, Familiari NE, Tran VM, Woodruff JB, Liem KF Jr, Mukhopadhyay S
Molecular biology of the cell 2023 Mar 1;34(3):ar18

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Multiple ciliary localization signals control INPP5E ciliary targeting.

Cilleros-Rodriguez D, Martin-Morales R, Barbeito P, Deb Roy A, Loukil A, Sierra-Rodero B, Herranz G, Pampliega O, Redrejo-Rodriguez M, Goetz SC, Izquierdo M, Inoue T, Garcia-Gonzalo FR
eLife 2022 Sep 5;11

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The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.

Schembs L, Willems A, Hasenpusch-Theil K, Cooper JD, Whiting K, Burr K, Bøstrand SMK, Selvaraj BT, Chandran S, Theil T
Cell reports 2022 May 17;39(7):110811

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INPP5E controls ciliary localization of phospholipids and the odor response in olfactory sensory neurons.

Ukhanov K, Uytingco C, Green W, Zhang L, Schurmans S, Martens JR
Journal of cell science 2022 Mar 1;135(5)

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Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via sonic hedgehog signaling.

Shi H, Wang H, Zhang C, Lu Y, Yao J, Chen Z, Xing G, Wei Q, Cao X
JCI insight 2022 Feb 22;7(4)

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The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic.

Turn RE, Hu Y, Dewees SI, Devi N, East MP, Hardin KR, Khatib T, Linnert J, Wolfrum U, Lim MJ, Casanova JE, Caspary T, Kahn RA
Molecular biology of the cell 2022 Feb 1;33(2):ar13

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Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E.

Dewees SI, Vargová R, Hardin KR, Turn RE, Devi S, Linnert J, Wolfrum U, Caspary T, Eliáš M, Kahn RA
Molecular biology of the cell 2022 Apr 1;33(4):ar33

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Down-Regulation of Inpp5e Associated With Abnormal Ciliogenesis During Embryonic Neurodevelopment Under Inositol Deficiency.

Yue H, Li S, Qin J, Gao T, Lyu J, Liu Y, Wang X, Guan Z, Zhu Z, Niu B, Zhong R, Guo J, Wang J
Frontiers in neurology 2021;12:579998

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ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.

Fujisawa S, Qiu H, Nozaki S, Chiba S, Katoh Y, Nakayama K
Biology open 2021 Sep 15;10(9)

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The Major Ciliary Isoforms of RPGR Build Different Interaction Complexes with INPP5E and RPGRIP1L.

Vössing C, Atigbire P, Eilers J, Markus F, Stieger K, Song F, Neidhardt J
International journal of molecular sciences 2021 Mar 30;22(7)

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Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Chen C, Xu Q, Zhang Y, Davies BA, Huang Y, Katzmann DJ, Harris PC, Hu J, Ling K
Science advances 2021 Jun;7(26)

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Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.

Luo M, Lin Z, Zhu T, Jin M, Meng D, He R, Cao Z, Shen Y, Lu C, Cai R, Zhao Y, Wang X, Li H, Wu S, Zou X, Luo G, Cao L, Huang M, Jiao H, Gao H, Sui R, Zhao C, Ma X, Cao M
Genetics in medicine : official journal of the American College of Medical Genetics 2021 Jun;23(6):1041-1049

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Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis.

Sharif AS, Gerstner CD, Cady MA, Arshavsky VY, Mitchell C, Ying G, Frederick JM, Baehr W
The Journal of biological chemistry 2021 Jan-Jun;296:100529

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Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry.

Qiu H, Fujisawa S, Nozaki S, Katoh Y, Nakayama K
Biology open 2021 Jan 25;10(1)

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Exocyst complex mediates recycling of internal cilia.

Rivera-Molina FE, Xi Z, Reales E, Wang B, Toomre D
Current biology : CB 2021 Dec 20;31(24):5580-5589.e5

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Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Hong JJ, Kim KE, Park SY, Bok J, Seo JT, Moon SJ
Molecules and cells 2021 Aug 31;44(8):591-601

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The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.

Boukhalfa A, Roccio F, Dupont N, Codogno P, Morel E
Cell reports 2021 Apr 27;35(4):109045

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Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway.

Gerakopoulos V, Ngo P, Tsiokas L
Life science alliance 2020 Sep;3(9)

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Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Wang W, Allard BA, Pottorf TS, Wang HH, Vivian JL, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381

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ARL13B regulates Sonic hedgehog signaling from outside primary cilia.

Gigante ED, Taylor MR, Ivanova AA, Kahn RA, Caspary T
eLife 2020 Mar 4;9

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The ciliary GTPase Arl3 maintains tissue architecture by directing planar spindle orientation during epidermal morphogenesis.

Bhattarai SR, Begum S, Popow R, Ezratty EJ
Development (Cambridge, England) 2019 May 10;146(9)

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Tulp3 Regulates Renal Cystogenesis by Trafficking of Cystoproteins to Cilia.

Hwang SH, Somatilaka BN, Badgandi H, Palicharla VR, Walker R, Shelton JM, Qian F, Mukhopadhyay S
Current biology : CB 2019 Mar 4;29(5):790-802.e5

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TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia.

Han S, Miyoshi K, Shikada S, Amano G, Wang Y, Yoshimura T, Katayama T
Biochemical and biophysical research communications 2019 Jan 29;509(1):227-234

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Disruption of RPGR protein interaction network is the common feature of RPGR missense variations that cause XLRP.

Zhang Q, Giacalone JC, Searby C, Stone EM, Tucker BA, Sheffield VC
Proceedings of the National Academy of Sciences of the United States of America 2019 Jan 22;116(4):1353-1360

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Development of a Molecularly Stable Gene Therapy Vector for the Treatment of RPGR-Associated X-Linked Retinitis Pigmentosa.

Giacalone JC, Andorf JL, Zhang Q, Burnight ER, Ochoa D, Reutzel AJ, Collins MM, Sheffield VC, Mullins RF, Han IC, Stone EM, Tucker BA
Human gene therapy 2019 Aug;30(8):967-974

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A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Frikstad KM, Molinari E, Thoresen M, Ramsbottom SA, Hughes F, Letteboer SJF, Gilani S, Schink KO, Stokke T, Geimer S, Pedersen LB, Giles RH, Akhmanova A, Roepman R, Sayer JA, Patzke S
Cell reports 2019 Aug 13;28(7):1907-1922.e6

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Characterization of Primary Cilia in Normal Fallopian Tube Epithelium and Serous Tubal Intraepithelial Carcinoma.

Abdelhamed ZA, Ryan TA, Fuller M, Coulson-Gilmer C, Abdelmottaleb DI, Wang TL, Kaun JC, Wang P, Hutson R, Wilkinson N, Bell SM, Johnson CA
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2018 Oct;28(8):1535-1544

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ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.

Alkanderi S, Molinari E, Shaheen R, Elmaghloob Y, Stephen LA, Sammut V, Ramsbottom SA, Srivastava S, Cairns G, Edwards N, Rice SJ, Ewida N, Alhashem A, White K, Miles CG, Steel DH, Alkuraya FS, Ismail S, Sayer JA
American journal of human genetics 2018 Oct 4;103(4):612-620

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The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling.

Goetz SC, Bangs F, Barrington CL, Katsanis N, Anderson KV
PloS one 2017;12(3):e0173399

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The Joubert Syndrome Protein Inpp5e Controls Ciliogenesis by Regulating Phosphoinositides at the Apical Membrane.

Xu W, Jin M, Hu R, Wang H, Zhang F, Yuan S, Cao Y
Journal of the American Society of Nephrology : JASN 2017 Jan;28(1):118-129

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KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.

Schou KB, Mogensen JB, Morthorst SK, Nielsen BS, Aleliunaite A, Serra-Marques A, Fürstenberg N, Saunier S, Bizet AA, Veland IR, Akhmanova A, Christensen ST, Pedersen LB
Nature communications 2017 Jan 30;8:14177

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Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E.

Nozaki S, Katoh Y, Terada M, Michisaka S, Funabashi T, Takahashi S, Kontani K, Nakayama K
Journal of cell science 2017 Feb 1;130(3):563-576

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The guanine nucleotide exchange factor Arf-like protein 13b is essential for assembly of the mouse photoreceptor transition zone and outer segment.

Hanke-Gogokhia C, Wu Z, Sharif A, Yazigi H, Frederick JM, Baehr W
The Journal of biological chemistry 2017 Dec 29;292(52):21442-21456

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Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

Fu W, Wang L, Kim S, Li J, Dynlacht BD
Cell reports 2016 Nov 1;17(6):1505-1517

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Inpp5e suppresses polycystic kidney disease via inhibition of PI3K/Akt-dependent mTORC1 signaling.

Hakim S, Dyson JM, Feeney SJ, Davies EM, Sriratana A, Koenig MN, Plotnikova OV, Smyth IM, Ricardo SD, Hobbs RM, Mitchell CA
Human molecular genetics 2016 Jun 1;25(11):2295-2313

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MKS1 regulates ciliary INPP5E levels in Joubert syndrome.

Slaats GG, Isabella CR, Kroes HY, Dempsey JC, Gremmels H, Monroe GR, Phelps IG, Duran KJ, Adkins J, Kumar SA, Knutzen DM, Knoers NV, Mendelsohn NJ, Neubauer D, Mastroyianni SD, Vogt J, Worgan L, Karp N, Bowdin S, Glass IA, Parisi MA, Otto EA, Johnson CA, Hildebrandt F, van Haaften G, Giles RH, Doherty D
Journal of medical genetics 2016 Jan;53(1):62-72

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Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

Xu Q, Zhang Y, Wei Q, Huang Y, Hu J, Ling K
Nature communications 2016 Feb 26;7:10777

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Deletion of Inpp5a causes ataxia and cerebellar degeneration in mice.

Yang AW, Sachs AJ, Nystuen AM
Neurogenetics 2015 Oct;16(4):277-85

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INPP5E interacts with AURKA, linking phosphoinositide signaling to primary cilium stability.

Plotnikova OV, Seo S, Cottle DL, Conduit S, Hakim S, Dyson JM, Mitchell CA, Smyth IM
Journal of cell science 2015 Jan 15;128(2):364-72

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A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.

Thomas S, Wright KJ, Le Corre S, Micalizzi A, Romani M, Abhyankar A, Saada J, Perrault I, Amiel J, Litzler J, Filhol E, Elkhartoufi N, Kwong M, Casanova JL, Boddaert N, Baehr W, Lyonnet S, Munnich A, Burglen L, Chassaing N, Encha-Ravazi F, Vekemans M, Gleeson JG, Valente EM, Jackson PK, Drummond IA, Saunier S, Attié-Bitach T
Human mutation 2014 Jan;35(1):137-46

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ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Humbert MC, Weihbrecht K, Searby CC, Li Y, Pope RM, Sheffield VC, Seo S
Proceedings of the National Academy of Sciences of the United States of America 2012 Nov 27;109(48):19691-6

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17797-1-AP(INPP5E antibody) at dilution of 1:1000

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 17797-1-AP(INPP5E antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 17797-1-AP(INPP5E antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue