Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF5329 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Plexin B2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Plexin B2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Plexin B2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Rnd3-induced cell rounding requires interaction with Plexin-B2.
McColl B, Garg R, Riou P, Riento K, Ridley AJ
Journal of cell science 2016 Nov 1;129(21):4046-4056
Journal of cell science 2016 Nov 1;129(21):4046-4056
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Plexin B2 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Plexin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5329) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin B2 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Plexin B2 in Human Colon Cancer Tissue. Plexin B2 was detected in immersion fixed paraffin-embedded sections of human colon cancer using 3 µg/mL Sheep Anti-Human Plexin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5329) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Plexin B2 in Mouse Brain. Plexin B2 was detected in perfusion fixed frozen sections of mouse brain (choroid plexus) using 10 µg/mL Sheep Anti-Human Plexin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5329) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.