Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Other assay [1]
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Validation data
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- Product number
- TA347131 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal H2A.XS139p Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal H2A.XS139p Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- H2AFX
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 2.75 ?g/?l
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB was performed on histone extracts (15 ug) from untreated U2OS cells (lane 1) or from U2OS cells treated with camptothecin (lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2A.XS139p. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.XS139p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:170,000.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- A Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2A.X. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Image shows a high specificity of the antibody for the modification of interest.
- Validation comment
- DB