TA347131

antibody from OriGene

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA347131

Provider

OriGene

Product name

Rabbit Polyclonal H2A.XS139p Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal H2A.XS139p Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

H2AFX

Isotype

IgG

Antibody clone number

NULL

Vial size

50 µg

Concentration

2.75 ?g/?l

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB was performed on histone extracts (15 ug) from untreated U2OS cells (lane 1) or from U2OS cells treated with camptothecin (lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2A.XS139p. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.XS139p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:170,000.

Validation comment

ELISA

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

A Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2A.X. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Image shows a high specificity of the antibody for the modification of interest.

Validation comment

DB