LS-C41114

antibody from LSBio

Targeting: H2AX H2AFX

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C41114

Provider

LSBio

Product name

H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) LS-C41114

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2F3

Storage

Store at 4°C. Store undiluted.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.