- GTX108272 - GeneTex H2AX antibody

Submitted references Ovatodiolide isolated from Anisomeles indica induces cell cycle G2/M arrest and apoptosis via a ROS-dependent ATM/ATR signaling pathways.

Yu CY, Jerry Teng CL, Hung PS, Cheng CC, Hsu SL, Hwang GY, Tzeng YM
European journal of pharmacology 2018 Jan 15;819:16-29

Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.

Liu MK, Lin JJ, Chen CY, Kuo SC, Wang YM, Chan HL, Wu TY
International journal of molecular sciences 2016 Jun 14;17(6)

The Thoc1 ribonucleoprotein and prostate cancer progression.

Chinnam M, Wang Y, Zhang X, Gold DL, Khoury T, Nikitin AY, Foster BA, Li Y, Bshara W, Morrison CD, Payne Ondracek RD, Mohler JL, Goodrich DW
Journal of the National Cancer Institute 2014 Nov;106(11)

Anti-cancer effect and gene modulation of ET-743 in human biliary tract carcinoma preclinical models.

Peraldo-Neia C, Cavalloni G, Soster M, Gammaitoni L, Marchiò S, Sassi F, Trusolino L, Bertotti A, Medico E, Capussotti L, Aglietta M, Leone F
BMC cancer 2014 Dec 5;14:918

Role of MRE11 in cell proliferation, tumor invasion, and DNA repair in breast cancer.

Yuan SS, Hou MF, Hsieh YC, Huang CY, Lee YC, Chen YJ, Lo S
Journal of the National Cancer Institute 2012 Oct 3;104(19):1485-502

A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species.

Chen HM, Chang FR, Hsieh YC, Cheng YJ, Hsieh KC, Tsai LM, Lin AS, Wu YC, Yuan SS
Free radical biology & medicine 2011 May 1;50(9):1151-62

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) 15% SDS PAGE GTX108272 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.A. 50 ?g mouse brain extract12% SDS-PAGEHistone H2A.X antibody (GTX108272) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.A. 50 ?g rat brain extract12% SDS-PAGEHistone H2A.X antibody (GTX108272) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HCT116 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of H2AFX protein by 4 £gg Histone H2A.X antibody (GTX108272)15 % SDS-PAGEThe immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H2A.X antibody (GTX108272) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H2A.X antibody (GTX108272) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H2A.X antibody (GTX108272) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus cancer. Histone H2A.X antibody (GTX108272) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Histone H2A.X antibody (GTX108272) diluted at 1:500.

GTX108272

Antibody data