TA319154

antibody from OriGene

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319154

Provider

OriGene

Product name

Rabbit polyclonal H2AX pS139 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal H2AX pS139 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

H2AFX

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 ug per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none.

Validation comment

WB