Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- R20231 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant phospho-Histone H2A/H4 Antibody (Ser1)
- Antibody type
- Recombinant
- Description
- Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM216
- Vial size
- 25 µg, 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant phospho-Histone H2A/H4 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot test of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using recombinant phospho-Histone H2A/H4 antibody at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF staining of HeLa cells using recombinant phospho-Histone H2A/H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- This recombinant phospho-Histone H2A/H4 antibody specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).