R20244

antibody from NSJ Bioreagents

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R20244

Provider

NSJ Bioreagents

Product name

Recombinant Gamma H2AX Antibody (phospho S139)

Antibody type

Recombinant

Description

Purified

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM224

Vial size

25 µg, 100 µg

Concentration

1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Storage

Store the recombinant Gamma H2AX antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot of acid extracts of HeLa cells treated or non-treated with UV, using recombinant Gamma H2AX antibody at 0.5 ug/ml, showed a band of Histone H2A.X phosphorylated at Serine 139 in HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC/IF of HeLa cells using recombinant Gamma H2AX antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).