Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-40152 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Histone H2A.X (Thr120) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of H2AFX in extracts from Hela cells, using an anti-H2AFX polyclonal antibody (Product # PA5-40152) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of H2A.X T120ph was performed by loading 10 µg of U2OS (lane 1) and NIH 3T3 chromatin bound nuclear lysates in reducing sample buffer (Product # 39000) and Page Ruler Plus Protein Ladder (Product # 26619) onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4201BX10). Proteins were transferred to nitrocellulose membrane (Product # 88018) with Transfer Buffer (Product # 84731) using the G2 Gast Blotter (Product # 62288). Membrane was blocked in StartingBlock T20 (Product # 37543) for one hour at room temperature. H2A.X T120ph was detected at approximately 15 kDa using a H2A.X T120ph polyclonal antibody (Product # PA5-40152) at a dilution of 1:1500 in StartingBlock T20 overnight at at 4°C on a rocking platform, followed by a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody (Product # A27036) at a dilution of 1:10,000 for one hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080) and the myECL Imager (Product # 62236).