Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ADI-KAM-CC255-D - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-KAM-CC255-D, RRID:AB_2039187
- Product name
- [pSer139]Histone H2AX monoclonal antibody (9F3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Protein G affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9F3
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references The bipartite rac1 Guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (elmo1/dock180) protects endothelial cells from apoptosis in blood vessel development.
Schäker K, Bartsch S, Patry C, Stoll SJ, Hillebrands JL, Wieland T, Kroll J
The Journal of biological chemistry 2015 Mar 6;290(10):6408-18
The Journal of biological chemistry 2015 Mar 6;290(10):6408-18
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of [pSer139] Histone H2AX, mAb (9F3) (Prod. No. ADI-KAM-CC255): Lane 1: MW marker; Lane 2: Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate.