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- Product number
- NB100-74435 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74435, RRID:AB_1048941
- Product name
- Mouse Monoclonal gamma H2AX Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. H2AX [Ser139] Phosphospecific (3F2) [Ser139]
- Reactivity
- Human, Mouse, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Enhanced Radiosensitivity in Solid Tumors using a Tumor-selective Alkyl Phospholipid Ether Analog.
Low-dose irradiation of mouse embryos increases Smad-p21 pathway activity and preserves pluripotency.
The tumor suppressor Hic1 maintains chromosomal stability independent of Tp53.
Chronic high fat feeding restricts islet mRNA translation initiation independently of ER stress via DNA damage and p53 activation.
Elsaid MY, Shahi A, Wang AR, Baiu DC, Li C, Werner LR, Singhal S, Hall LT, Weichert JP, Armstrong EA, Bednarz BP, Harari PM, Iyer G, Otto M
Molecular cancer therapeutics 2018 Nov;17(11):2320-2328
Molecular cancer therapeutics 2018 Nov;17(11):2320-2328
Low-dose irradiation of mouse embryos increases Smad-p21 pathway activity and preserves pluripotency.
Hayashi M, Yoshida K, Kitada K, Kizu A, Tachibana D, Fukui M, Morita T, Koyama M
Journal of assisted reproduction and genetics 2018 Jun;35(6):1061-1069
Journal of assisted reproduction and genetics 2018 Jun;35(6):1061-1069
The tumor suppressor Hic1 maintains chromosomal stability independent of Tp53.
Szczepny A, Carey K, McKenzie L, Jayasekara WSN, Rossello F, Gonzalez-Rajal A, McCaw AS, Popovski D, Wang D, Sadler AJ, Mahar A, Russell PA, Wright G, McCloy RA, Garama DJ, Gough DJ, Baylin SB, Burgess A, Cain JE, Watkins DN
Oncogene 2018 Apr;37(14):1939-1948
Oncogene 2018 Apr;37(14):1939-1948
Chronic high fat feeding restricts islet mRNA translation initiation independently of ER stress via DNA damage and p53 activation.
Hatanaka M, Anderson-Baucum E, Lakhter A, Kono T, Maier B, Tersey SA, Tanizawa Y, Evans-Molina C, Mirmira RG, Sims EK
Scientific reports 2017 Jun 19;7(1):3758
Scientific reports 2017 Jun 19;7(1):3758
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Analysis of phosphorylated H2AX in gamma irradiated HeLA cell lysate. Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 10 ug/ml dilution on Jurkat lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Analysis of gamma H2AX antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Figure 1 illustrates WB analysis of phosphorylated H2AX in gamma irradiated Hela cell lysate. Lane 1: Unphosphorylated H2AX control Lane 2: Phosphorylated H2AX. Figure 2 shows an IF image of phosphorylated H2AX in gamma irradiated Hela cells.
