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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- RQ5506 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PPCS Antibody / Phosphopantothenoylcysteine synthetase
- Antibody type
- Monoclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antibody clone number
- 7G13
- Vial size
- 100 ug
- Storage
- After reconstitution, the PPCS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A375 and 2) COLO-320 cell lysate with PPCS antibody. Predicted molecular weight ~34 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse heart with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat heart with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human PC-3 cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.
