NB100-74602
antibody from Novus Biologicals
Targeting: KDM2A
CXXC8, DKFZP434M1735, FBL11, FBL7, FBXL11, FLJ00115, JHDM1A, KIAA1004, LILINA
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-74602 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74602, RRID:AB_1048752
- Product name
- Rabbit Polyclonal KDM2A/FBXL11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references HPV16 E7-induced upregulation of KDM2A promotes cervical cancer progression by regulating miR-132-radixin pathway.
Transcriptional repression of histone deacetylase 3 by the histone demethylase KDM2A is coupled to tumorigenicity of lung cancer cells.
KDM2A promotes lung tumorigenesis by epigenetically enhancing ERK1/2 signaling.
Ou R, Zhu L, Zhao L, Li W, Tao F, Lu Y, He Q, Li J, Ren Y, Xu Y
Journal of cellular physiology 2019 Mar;234(3):2659-2671
Journal of cellular physiology 2019 Mar;234(3):2659-2671
Transcriptional repression of histone deacetylase 3 by the histone demethylase KDM2A is coupled to tumorigenicity of lung cancer cells.
Dhar SS, Alam H, Li N, Wagner KW, Chung J, Ahn YW, Lee MG
The Journal of biological chemistry 2014 Mar 14;289(11):7483-96
The Journal of biological chemistry 2014 Mar 14;289(11):7483-96
KDM2A promotes lung tumorigenesis by epigenetically enhancing ERK1/2 signaling.
Wagner KW, Alam H, Dhar SS, Giri U, Li N, Wei Y, Giri D, Cascone T, Kim JH, Ye Y, Multani AS, Chan CH, Erez B, Saigal B, Chung J, Lin HK, Wu X, Hung MC, Heymach JV, Lee MG
The Journal of clinical investigation 2013 Dec;123(12):5231-46
The Journal of clinical investigation 2013 Dec;123(12):5231-46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74602 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. JHDM1A was also immunoprecipitated by another rabbit anti-JHDM1A antibodies and NB100-74603, which recognize other epitopes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of Human JHDM1A by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug) and 293T (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody NB100-74602 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of human JHDM1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody NB100-74602 (lot NB100-74602-2) used for IP at 6 ug per reaction. JHDM1A was also immunoprecipitated by a previous lot of this antibody (lot NB100-74602-1). For blotting immunoprecipitated JHDM1A, NB100-74602 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - Analysis of human lung tumor tissue using anti-KDM2A antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- JHDM1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB