TA501121

antibody from OriGene

Targeting: NIT2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501121

Provider

OriGene

Proper citation

OriGene Cat#TA501121, RRID:AB_11128584

Product name

Anti-NIT2 mouse monoclonal antibody, clone OTI5A3 (formerly 5A3)

Antibody type

Monoclonal

Description

Anti-NIT2 mouse monoclonal antibody, clone OTI5A3 (formerly 5A3)

Host

Mouse

Conjugate

Unconjugated

Epitope

NIT2

Isotype

IgG

Antibody clone number

OTI5A3

Vial size

100 µl

Concentration

0.95 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-NIT2 mouse monoclonal antibody (TA501121) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HT29 cells using anti-NIT2 mouse monoclonal antibody (TA501121).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501121, Dilution 1:50)

Validation comment

IHC