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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- AF3258 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Siglec-11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Siglec-11 in direct ELISAs and Western blots. In direct ELISA and Western blots, approximately 20% cross-reactivity with recombinant human (rh) Siglec-10 is observed and less than 5% cross-reactivity with rhSiglec-2, -3, -5, -6, -7, -9 and recombinant mouse Siglec-F is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Siglec-11 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes (PBMC) were labeled with Goat Anti-Human Siglec-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3258) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).
